AustralieFrV1, Main, Exploration, bibRecord, 009568

Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract

Identifieur interne : 009568 ( Main/Exploration ); précédent : 009567; suivant : 009569

Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract

Auteurs : Glen C. Ulett [Australie] ; Jaione Valle [France] ; Christophe Beloin [France] ; Orla Sherlock [Australie] ; Jean-Marc Ghigo [France] ; Mark A. Schembri [Australie]

Source :

Infection and immunity [ 0019-9567 ] ; 2007.

RBID : Pascal:07-0361242

Descripteurs français

Pascal (Inist)
- Escherichia coli, Antigène, Souche uropathogène.

English descriptors

KwdEn :
- Antigen, Escherichia coli.

Abstract

Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932929

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract</title>
<author><name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0361242</idno>
<date when="2007">2007</date>
<idno type="stanalyst">PASCAL 07-0361242 INIST</idno>
<idno type="RBID">Pascal:07-0361242</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">003A94</idno>
<idno type="wicri:Area/PascalFrancis/Curation">002594</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">003881</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">003881</idno>
<idno type="wicri:doubleKey">0019-9567:2007:Ulett G:functional:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">009F93</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932929</idno>
<idno type="RBID">PMC:1932929</idno>
<idno type="wicri:Area/Pmc/Corpus">000F93</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F93</idno>
<idno type="wicri:Area/Pmc/Curation">000E54</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E54</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002B20</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002B20</idno>
<idno type="wicri:Area/Ncbi/Merge">000259</idno>
<idno type="wicri:Area/Ncbi/Curation">000259</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000259</idno>
<idno type="wicri:doubleKey">0019-9567:2007:Ulett G:functional:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">009970</idno>
<idno type="wicri:Area/Main/Curation">009568</idno>
<idno type="wicri:Area/Main/Exploration">009568</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract</title>
<author><name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Groupe de genetique des biofilms, URA CNRS 2172, Institut Pasteur, 25, rue du Dr Roux</s1>
<s2>75724 Paris</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>75724 Paris</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1>
<s2>Brisbane, QLD 4072</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, QLD 4072</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Infection and immunity</title>
<title level="j" type="abbreviated">Infect. immun.</title>
<idno type="ISSN">0019-9567</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Infection and immunity</title>
<title level="j" type="abbreviated">Infect. immun.</title>
<idno type="ISSN">0019-9567</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigen</term>
<term>Escherichia coli</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Escherichia coli</term>
<term>Antigène</term>
<term>Souche uropathogène</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter subgroup of proteins. The best characterized of these proteins, antigen 43 (Ag43), is a self-recognizing adhesin that is associated with cell aggregation and biofilm formation in E. coli K-12. The sequenced genome of prototype UPEC strain CFT073 contains two variant Ag43-encoding genes located on pathogenicity islands. The biological significance of both of these genes and their role in UPEC pathogenesis have not been investigated previously. Here we performed a detailed molecular characterization analysis of Ag43a (c3655) and Ag43b (c1273) from UPEC CFT073. Expression of Ag43a and Ag43b in a K-12 background revealed that they possess different functional properties. Ag43a produced a strong aggregation phenotype and promoted significant biofilm growth. Deletion mutants and strains constitutively expressing Ag43a and Ag43b were also constructed using CFT073. When these mutants were analyzed in a mouse model of UTI, Ag43a (but not Ag43b) promoted long-term persistence in the urinary bladder. Our findings demonstrate that Ag43a contributes to UPEC disease pathogenesis and reveal that there are pathogenicity-adapted variants of Ag43 with distinct virulence-related functions.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name>
</noRegion>
<name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name>
<name sortKey="Sherlock, Orla" sort="Sherlock, Orla" uniqKey="Sherlock O" first="Orla" last="Sherlock">Orla Sherlock</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name>
</region>
<name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name>
<name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 009568 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 009568 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:07-0361242
   |texte=   Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

Serveur d'exploration sur les relations entre la France et l'Australie

Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract

Functional analysis of antigen 43 in uropathogenic Escherichia coli reveals a role in long-term persistence in the urinary tract

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.